Nickel Carcinogenesis Mechanism: DNA Damage
After DNA damage occurs, cells activate several response signals, such as cell-cycle arrest, DNA repair, and cell death [ 26, 27, 28 ]. Numbers in in vitro and in …
DNA methylation mediates the effects of PM2.5 and O3 on …
Mediation analysis was subsequently performed to evaluate the potential mediating effect of DNA methylation in the association between air pollution and ceramide metabolism. PM 2.5 and O 3 were associated with elevated IR. Specifically, each 10 μg/m 3 increase in PM 2.5 and O 3 at lag0–12 h significantly increased triglyceride‑glucose ...
Effects of prenatal exposure to (es)citalopram and maternal …
Studies assessing associations between prenatal exposure to antidepressants, maternal depression, and offspring DNA methylation (DNAm) have been inconsistent. Here, we investigated whether prenatal exposure to citalopram or escitalopram ((es)citalopram) and maternal depression is associated with dif …
Effects of nickel on DNA methyltransferase activity and genomic DNA
These results suggest that nickel exposure can elevate total genomic DNA methylation levels even when DNA methyltransferase activity is depressed. These findings may explain the hypermethylation of senescence and tumor suppressor genes found during nickel carcinogenesis and support the model of a direct effect of Ni2+ on chromatin …
The effects of nickel (II) complexes with imidazole …
The effects of nickel(II) complexes with 1-allylimidazole or imidazole-4-acetate anion, and their ligands alone, on P. aeruginosa growth in correlation with pyoverdine production. White bars ...
Effect of nickel(II) on DNA-protein interactions
14 Citations. Explore all metrics. Abstract. Alterations in DNA-protein interactions (DPI) may play an important role in carcinogenesis. Although the mechanism of nickel …
Oxidative modification enhances the immunostimulatory effects …
One of these endogenous danger signals is the extracellular mitochondrial DNA (mtDNA) containing evolutionary conserved unmethylated CpG repeats. ... (HLA-DQ) on the cell surface, as well as increased TNF-α and IL-8 production from the cells. These effects were more apparent when pDCs were exposed to oxidatively modified mtDNA. Neither native ...
Repair and biochemical effects of DNA-protein crosslinks
However, various endogenous, environmental, and chemotherapeutic agents induce DNA-protein crosslinks (DPCs), and hence covalently trap proteins on DNA. Since DPCs are extremely large compared to conventional DNA lesions, they probably impair many aspects of DNA transactions such as replication, transcription, and repair due to …
Effect of nickel chloride on seed germination and
The effect of nickel chloride concentrations in the range of 1–1000 mg/L on seed germination and the development of organs and pigment system in seedlings of the helophyte common water-plantain Alisma plantago-aquatica L. and hygrohelophyte greater water-parsnip Sium latifolium L. has been studied. It is shown that nickel chloride has …
Effect of nickel(II) on the specific binding of proteins …
Effect of nickel (II) on the specific binding of proteins to UV- irradiated DNA and protective interaction of magnesium (II). HeLa cells were incubated for 24 h with the respective...
A systematic review and meta-analysis to determine the effect
Sperm DNA damage is prevalent among infertile men and is known to influence natural reproduction. However, the impact of sperm DNA damage on assisted reproduction outcomes remains controversial. Here, we conducted a meta-analysis of studies on sperm DNA damage (assessed by SCSA, TUNEL, SCD, or Comet …
Exploring the Molecular Mechanisms of Nickel …
Experimental studies have shown that nickel disrupts DNA strands, DNA crosslinks, and DNA repair. Oxidative stress has been found to play a key role in nickel-induced carcinogenicity / 47, 48 /. The production of reactive oxygen species (ROS) is …
Effects of nickel(II) on nuclear protein binding to …
Since regulation of DNA replication and gene expression is dependent upon DNA-protein interactions, the effect of nickel in altering the extent of DNA-protein binding may …
Position-dependent effects of locked nucleic acid (LNA) on DNA
Locked Nucleic Acid (LNA) can enhance oligonucleotide affinity and specificity. Though LNA has been used in many applications, formal design rules are still being defined. To further this effort we have investigated the effect of LNA on the performance of sequencing and PCR primers in AT-rich regions, where short primers yield poor sequencing ...
Effects of DNA supercoiling on chromatin architecture
We propose a model in which changes in unconstrained DNA supercoiling influences higher levels of chromatin organisation through the additive effects of DNA supercoiling on both DNA-protein and DNA-nucleosome interactions. This model links small-scale changes in DNA and chromatin to the higher-order fibre and large-scale chromatin structures ...
Nickel(II) Chloride
Nickel is absorbed through the lungs, gastrointestinal tract, and skin. Water-soluble nickel compounds (e.g., nickel chloride or sulfate) are absorbed better than insoluble nickel is. Approximately 25% of the nickel in drinking water is absorbed. Nickel applied to the skin is absorbed into the skin but may not reach the circulation.
Exploring the binding and cleavage activities of nickelII …
DOI: 10.1039/D0NJ06210C Corpus ID: 234170238; Exploring the binding and cleavage activities of nickelII complexes towards DNA and proteins @article{Kumar2021ExploringTB, title={Exploring the binding and cleavage activities of nickelII complexes towards DNA and proteins}, author={Manish Kumar and Neetika Lal …
DNA intermediates at the Escherichia coli replication fork: effect …
We have directly tested the hypothesis that elevated levels of dUTP cause the formation of small DNA fragments at the replication fork of Escherichia coli. Addition of increasing levels of dUTP to lysates on cellophane discs results in an increasing number of strand scissions in the newly replicated DNA. Lysates of strains defective in dUTPase ...
Effect of Temperature on the Intrinsic Flexibility of DNA …
Double-stranded DNA (dsDNA) is a semiflexible polymer. 1 On short length scales (on the order of its persistence length, ∼150 bp or ∼50 nm), stiffness dominates its conformation and bending is energetically unfavorable. However, on a larger scale, dsDNA acts as a flexible polymer and forms a random coil. Although the exact cause of DNA …
Effect of mechanical stretching on DNA conductance
Here we study the effect of mechanical stretching-induced structural changes on charge transport in single DNA molecules. The charge transport follows the hopping mechanism for DNA molecules with lengths varying from 6 to 26 base pairs, but the conductance is highly sensitive to mechanical stretching, showing an abrupt decrease at …
Lingering effects of Neanderthal DNA found in modern …
Lingering effects of Neanderthal DNA found in modern humans. Recent scientific discoveries have shown that Neanderthal genes comprise some 1 to 4% of the genome of present-day humans whose ancestors migrated out of Africa, but the question remained open on how much those genes are still actively influencing human traits — …
Exploring the binding and cleavage activities of nickelII …
The present study documents the first investigation into the relationship between the binding and cleavage activity of nickel complexes towards DNA and proteins. Here, three different NiII complexes of the general type [Ni(nal)2diimine] (1a and 1b) and [Ni(8-Hq)2(bpy)] 1c (nal = nalidixic acid, diimine = 2,2
Dissecting the pathogenic effects of smoking and its …
Background: Tobacco smoking is suggested as a risk factor for colorectal cancer (CRC), but the complex relationship and the potential pathway are not fully understood. Methods: We performed two-sample Mendelian randomisation (MR) analyses with genetic instruments for smoking behaviours and related DNA methylation in blood …
The Effects of RNA.DNA-DNA Triple Helices on Nucleosome …
Biochemical experiments have shown that ncRNA binding to nucleosomal DNA is elevated at DNA entry and exit sites and is dependent on the presence of the H3 N-terminal tails. However, the structural and dynamical bases for these mechanisms remains unknown. Here, we have examined the mechanisms and effects of RDD formation in the context of …
Effect of Aphidicolin, a Reversible Inhibitor of Eukaryotic Nuclear DNA
There is no previous information on the use of aphidicolin in porcine embryos, so the reversible inhibition of DNA replication and the effect on embryo development of different concentrations of this drug was first evaluated. The effect of incubation with aphidicolin was tested with CRISPR/Cas9 at different concentrations and …
Effects of nickel(II) on nuclear protein binding to …
An intracellular effect of nickel(II) which may be involved in its carcinogenic action is the alteration of normal DNA-protein binding. This effect of ionic nickel was studied in …
The effects of tissue fixation alternatives on DNA content: …
Abstract. The aim of this study is to investigate the effects of different fixatives on DNA, and to evaluate the fixation options for molecular studies including polymerase chain reaction (PCR) and fluorescence in-situ hybridization (FISH). Three normal-looking colonic segments from surgical resections were used for tissue sampling.
4,4,4-Trifluoro-1-(thiophen-2-yl)butane-1,3-dione nickel(II) …
Docking studies on DNA complexes confirm the binding of 1 and 2 in the major groove of CT-DNA (CTP-1 Endonuclease). Moreover, the antibacterial effect of 1-3 against the five bacterial species was ...
The Effects of Dithiothreitol on DNA
DTT was furthermore shown to facilitate the immobilization of fluorescent DNA on an NHS-ester functionalized glass surface. Such reactions may in particular impact the readout from single molecule detection studies and other ultrasensitive assays. This was highlighted by the finding that DTT markedly decreased the signal to noise ratio in a …
Formation, Repair, and Biological Effects of DNA–Protein Cross …
In DNA repair, NER is the major mechanism for the repair of conventional bulky lesions. NER exhibits a robust activity for DNA–peptide cross-links, but a poor to negligible activity for DPCs. The initial recognition of DPCs by NER factors appears to be critical, and is compromised due to the steric hindrance of DPCs.
